The problem of treating diseases of affluence is now a serious challenge for modern medicine. Many research centers focus their efforts on the development of new experimental therapies including protein therapy, which focuses on intracellular use of therapeutics such as peptides and proteins. To be effective the proposed biomolecules must have an element which helps them penetrate the cell membrane. An example of such an element is polyarginine transporting peptide (PTP). The production of biotherapeutics with PTP in heterologous systems entails their purification prior to any final application.

In our studies we have developed a unique alternative to the ion-exchange chromatography (IEX) method for PTP tagged protein purification. It is based on reversible binding of PTP by single-stranded DNA (DNA aptamer). In comparision to IEX, this technology has two major advantages –

  1. specific binding of PTP-biomolecules to the DNA aptamer and
  2. efficient elution of the purified protein from the chromatography resin.

We estimate that the time necessary for the introduction of this technology into the global market is around two years.

Our main goal is the production of a commercially available chromatography resin for the purification of polyarginine-tagged peptides, proteins and other biomolecules. To achieve this aim the following development stages must be completed:

 1. Improved stability – the stability of anti-arginine tag aptamers containing natural or modified nucleotides (resistant to DNAse treatment) must be tested and compared.

 2. Immobilization of the anti-polyarginine tag aptamer on the appropriate carrier used in protein chromatography e.g. cellulose, agarose etc.

 We are searching for partners interested in the development of this chromatography system and its introduction on the market.


Meet the presenter:

FILIP BARTNICKI, PhD student (Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University)

Contact: tel. +48 603-197-029; email: f.bartnicki [at ] uj.edu.pl