Modern research methods

Microscopic techniques are routinely used in histopathological diagnostics and microbiological analyzes. Thanks to the development of new research methodology and the use of quantitative analysis of microscopic images, it is possible to conduct highly sophisticated studies that allow, for example, to assess the effect of therapy or various types of substances on the functions of single cells and the whole organisms. Confocal microscopy is a versatile imaging technique that enables three-dimensional high-resolution observations of fixed and live samples. At the ŁUKASIEWICZ − PORT Bioimaging Laboratory, we focus on analyzes of new therapeutic agents and biocompatible materials in various experimental models, both in vitro and in vivo. For example, one of the challenges of modern medicine are bacterial biofilms that form on surfaces of materials used in implants. Using the microscopic LIVE / DEAD test, we are able to help in choosing the right materials for clinical use by testing biofilm formation on metal implant screws. We can also use a microscopic approach to assess the safety of new biomaterials used to stimulate wound healing. By imaging cells of the immune system, we can measure stress-induced production of reactive oxygen species in response to biomaterials and their by-products including bacterial contaminations. We also analyze more complex tissue systems, measuring, for example, the permeability of substances through the skin, or conduct in vivo tests on laboratory animals by testing the effect of new oncological therapies on the distribution and the activity of immune cells. Our activities prove that confocal microscopy combined with quantitative image analysis can be used in many tests that can be offered as a service to business partners interested in developing new therapeutic agents.

Nowadays, the focus on reduction and cost optimization is of great importance, which is why the outsourcing of R&D services is becoming increasingly popular and many companies use it. Taking advantage of its rich research resources, ŁUKASIEWICZ − PORT offers services for the pharmaceutical and biotechnology industry. ŁUKASIEWICZ − PORT 's offer includes preclinical studies of new therapies consisting of in vivo tests (laboratory mice, Galleria mellonella) and full cytometric, histological and microscopic analysis (confocal, electron scanning microscopy) of collected research samples. In addition, we carry out cytotoxicity tests of various substances (MTT test) and genotoxicity (Ames test). We offer services of the production and purification of proteins, antibodies and conjugates as well as quantitative and qualitative characteristics of proteins: identification, structural analysis, purity and activity. In addition, Biobank operating at ŁUKASIEWICZ − PORT is a unit that coordinates biobanking in Poland. As part of the activity, biological material from healthy patients and with specific disease entities is collected and characterized. We invite you to cooperate with ŁUKASIEWICZ − PORT.

Monika Toporkiewicz
Łukasiewicz Research Network - PORT Polish Center for Technology Development

Research Engineer in the Bioimaging Laboratory, where she is responsible for the implementation of scientific projects and commercial orders. He is a biotechnologist by profession. She completed doctoral studies in Molecular Biology at the University of Wrocław. During her doctoral studies, she conducted research on liposomal carriers of genetic drugs directed by means of antibodies in the treatment of blood cancer. Her field of interest includes 3D cultures of skin cells and high-throughput tests of new compounds with antitumor activity based on fluorescence microscopy.